evaluation of metallo-β-lactamase-production and carriage of bla-vim genes in pseudomonas aeruginosa isolated from burn wound infections in isfahan
نویسندگان
چکیده
conclusions our findings showed a high occurrence of metallo-β-lactamase production among p. aeruginosa isolates in burn patient infections in our region. also, there are p. aeruginosa isolates carrying the bla-vim-1 and bla-vim-2 genes in isfahan province. background metallo-β-lactamase-production among gram-negative bacteria, including pseudomonas aeruginosa, has become a challenge for treatment of infections due to these resistant bacteria. objectives the aim of the current study was to evaluate the metallo-β-lactamase-production and carriage of bla-vim genes among carbapenem-resistant p. aeruginosa isolated from burn wound infections. patients and methods a cross-sectional study was conducted from september 2014 to july 2015. one hundred and fifty p. aeruginosa isolates were recovered from 600 patients with burn wound infections treated at imam-musa-kazem hospital in isfahan city, iran. carbapenem-resistant p. aeruginosa isolates were screened by disk diffusion using clsi guidelines. metallo-β-lactamase-producing p. aeruginosa isolates were identified using an imipenem-edta double disk synergy test (edta-imp ddst). for detection of mbl genes including bla-vim-1 and bla-vim-2, polymerase chain reaction (pcr) methods and sequencing were used. results among the 150 p. aeruginosa isolates, 144 (96%) were resistant to imipenem by the disk diffusion method, all of which were identified as metallo-β-lactamase-producing p. aeruginosa isolates by edta-imp ddst. twenty-seven (18%) and 8 (5.5%) mbl-producing p. aeruginosa isolates harbored bla-vim-1 and bla-vim-2 genes, respectively.
منابع مشابه
Evaluation of Metallo-β-Lactamase-Production and Carriage of bla-VIM Genes in Pseudomonas aeruginosa Isolated from Burn Wound Infections in Isfahan
BACKGROUND Metallo-β-lactamase-production among Gram-negative bacteria, including Pseudomonas aeruginosa, has become a challenge for treatment of infections due to these resistant bacteria. OBJECTIVES The aim of the current study was to evaluate the metallo-β-lactamase-production and carriage of bla-VIM genes among carbapenem-resistant P. aeruginosa isolated from burn wound infections. PATI...
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archives of trauma researchجلد ۵، شماره ۴، صفحات ۰-۰
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